Gas Chromatography Mass Spectrometry Unite two powerful methods to have the ability to extend the identification of materials which have low detection limits and potential required for quantitative analysis. Gas Chromatography Mass Spectrometry analyses works on fluids, solid and gaseous samples but are primarily restricted to semi volatile and volatile substances. Under Gas Chromatography, a sample is carried and volatilized and carried in the inter gases by means of a coated glass Capillary Column. The stationary phase is combined into the interior of the column and the amount of time it takes a specific compound of an element to maneuver through this column into a sensor is called its Retention Time. These measurements are used for identification purpose when compared to references.
Under ordinary Mass Spectrometry or MS Step beneath the Gas Chromatography Mass Spectrometry, the substances leaving the GC columns are fragmented by the electron impact. These charged fragments are detected gradually and the next spectrum obtained is used to identify the molecules. Fragmentation patterns are reproducible and consequently, can be used to make quantitative measurements. Gas Chromatography Mass Spectrometry analysis is done on fluids, gases and solids. For fluids and gases, the samples are usually injected directly into the Gas Chromatogram. For the solid elements, the analysis is carried us via outgassing or desorption, solvent extraction or pyrolysis. Desorption experimentation is done under the flow of helium gas at controlled temperatures ranging from 40 to 300 degree Celsius. The analytes are then collected on a Cryogenic trap during desorption procedure. The sample chamber is a 1.25×4 cylinder.
Pyrolysis is Another sampling Technique employed for the analysis of materials which are impossible to be directly injected into gas chromatography Mass Spectrometry. Under this process, heat is directly applied to the sample that Contributes to breaking down of those molecules in a reproducible way. These Smaller molecules are then introduced in the Gas Chromatogram and Examined in the GC-MS. By this procedure, probe temperatures of approximately 1400ºC may be used. Several other sampling methods and sample preparation are available such as static headspace analysis, derivatization, purge and trap, Solid Phase Microextraction etc. GC MS has long been Thought of as the Gold Standard for All sorts of analytical laboratories. It is a powerful analytical tool that Uses time to acquire the chemical components to travel across the GC column, the retention period in contrast to known standards to identify the chemical.